Researchers explain how railways within cells are built in order to transport essential cargos
Complex system transports essential cargoes such as proteins and membrane vesicles
Every cell in the human body contains a complex system to transport essential cargoes such as proteins and membrane vesicles, from point A to point B. These tiny molecular motor proteins move at blistering speeds on miniature railways carrying components of the cell to their proper destinations. But just how cells construct these transport railways to fit precisely inside of confined spaces of the individual cells has been a complex question, as it is critical that these railways do not grow too long or come up too short, as that would cause a misdirection of the proteins being transported.
Bruce Goode, professor of biology, working in collaboration with the labs of Laurent Blanchoin (Grenoble, France) and Roland Wedlich-Soldner (Munich, Germany), have come one step closer to understanding the elusive mechanics of this process.
In a recent paper published in Developmental Cell, a team led by Goode's Ph.D. student Melissa Chesarone-Cataldo shows that the length of the railways is controlled by one of its "passengers," which pauses during the journey to communicate with the machinery that is building the railways.
Enter Smy1, a myosin-passenger protein.
Goode and his colleagues hypothesized that a passenger protein like Smy1 would provide the perfect mechanism for slowing down formins when roadways are longer and would be carrying more passengers. They tested their theory in yeast cells, where formins construct actin cables that transport building materials essential for cell growth and division. As Goode says, they struck gold.
When they deleted the gene for Smy1, cables grew abnormally fast and hit the back of the cell, buckling and misdirecting transport. When they purified Smy1 and placed it in a test tube with formins they discovered that Smy1 slows down actin filament growth.
To further explore, they tagged Smy1 in living cells and learned that Smy1 molecules are carried on cables by myosin to the formin, where they pause for 1-2 seconds to give formins the message to slow down.
Goode says their working model illustrates that as a cable grows longer, it loads up more and more Smy1 molecules, which are transported on the cable to send a message to the formin to slow down.
"This prevents overgrowth of longer cables that are nearing the back of the cell, but allows rapid growth of the shorter cables," says Goode.
This paper will help scientists understand the general mechanisms that are used for directing cell shape and division. The next challenge says Goode, is "to find out whether related mechanisms are used to control formins in mammalian cells and understand the physiological consequences of disrupting those mechanisms."
Contact: Susan Chaityn Lebovits firstname.lastname@example.org 781-736-4027 Brandeis University